Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling.

  • Stirling, William, 1851-1932.
Date:
1902
Catalogue details

Licence: Public Domain Mark

Credit: Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling. Source: Wellcome Collection.

    100/690 (page 70)
    I. W.L. of absorption bands of oxy-haemoglobin.— (a) Arrange appar¬ atus as shown in fig. 33. A is the telescope through which the observer looks at the spectrum obtained by the light passing through B, and dis¬ persed by the flint-glass prism in the eentre of the apparatus. In C is fixed a scale photographed on glass and illuminated by a fan-tailed burner. D is the hcematinometer containing the dilute blood. (b) Place black velvet over the prism ; light both lamps ; look through A; adjust the slit in B, and the telescope in A, so as to get a good view of the spectrum, and over it the image of the scale. D is supposed not to be in position at first. On platinum wire, burn NaCl in the flame to get the yellow sodium line D. Adjust the scale so that this line corresponds to the figures 58 -9 on the scale, and fix the spectroscope tubes (A and C) in this position ; the scale is now accurately adjusted for all other parts of the spectrum. Fig. 33.—Arrangement of the Spectroscope for determining wave-lengths.—A, Telescope. B, Collimator tube. C, Scale tube. D, Hsematinometer. “ The numbers on the scale indicate wave-lengths expressed in one hundred thousandths of a millimetre, and each division indicates a differ¬ ence in wave-length equal to one hundred thousandth of a millimetre” (Gamgee). Thus, Fraunhofer’s line, D, which corresponds to division 58*9 of the scale, has a wave-length of 589 millionths of a millimetre. The wave¬ lengths of Fraunhofer’s lines are:—A = 760-4, B = 687-4, C = 656-7, D = 589-4, E = 527-3, F - 486-5. . (c) Using a blank map supplied with Zeiss’s spectroscope — the maps correspond to the scale seen in the spectroscope—fill in, in wave-lengths, the position of Fraunhofer’s lines B to F.