Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling.

  • Stirling, William, 1851-1932.
Date:
1902
Catalogue details

Licence: Public Domain Mark

Credit: Outlines of practical physiology : being a manual for the physiological laboratory, including chemical and experimental physiology, with reference to practical medicine / by William Stirling. Source: Wellcome Collection.

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    (b) Shake (a) oxy-alkali-haematin with air. Its spectrum shows one absorption-band just to the red side of D, but nearer D than that of acid hsematin (fig. 23). Much of the blue end of the spectrum is cut off. III. Reduced alkali-hsematin or haemochromogen.—(a) Add to alkali-hsematin a few drops of (NH4)2S, and heat gently. Note the change of colour = reduced alkali-hsematin, Stokes’ reduced hsematin or haemochromogen. The spectrum shows two absorp¬ tion-bands in the green between D and E (Hb02 and HbCO), but they are nearer the violet. The first band to the violet side of D is well defined ; the second, still nearer the violet (in fact, it nearly coincides with the E line), is less defined. Both bands disappear after shaking with air, and re-appear on standing, provided sufficient (NH4)2S be added. IV. Haematoporphyrin in acid solution (Iron-free hsematin, C16H1SN20).—(a) To strong H2S04 add a few drops of undiluted blood (about 5 drops of blood to 8 to 10 c.c. H2S04); mix, when a clear violet-red or purple-red fluid is obtained. Smell, odour of “ halitus sanguinis.” If black specks of carbon appear, filter through asbestos. Dilute only with concentrated H2S04, not h2o. (6) Observe two absorption-bands, one narrow, close to and on the red side of D, and a second half-way between D and E. (c) To the violet-red solution add an excess of water, which throws down part of the hasmatoporphyrin as a brown precipitate, which is more copious if the acid be neutralised with an alkali, e.g., NaHO. Dissolve some of the deposit in NaHO, and examine it spectroscopically. (d) The spectrum shows four absorption-bands ; a faint band midway between C and D, another similar one between D and E, but close to D; a third band near E ; and a fourth one, darkest of all, occupying the greater part of the space between b and F, but nearer the former. Picro-carmine.—Its spectrum closely resembles that of Hb02, but the two bands are much nearer the violet, one being midway between D and E, and the other to the violet side of E. The bands are unchanged on addi¬ tion of (NH4)2S or Stokes’ fluid. The solution does not give proteid reactions. Try also carmine, magenta, hsematoxylin, saffranin, eosin. V. Detection of blood-stains—e.g., rag stained with blood. (а) Add glycerin to part of the stain, and after a time express the liquor, and observe it microscopically for blood-corpuscles. (б) Suspend in distilled water by means of a thread a part of the rag, and leave it until the colouring-matter is extracted. Observe the coloured fluid spectroscopically. (c) Boil some of the extract with HC1, and add potassium ferrocyanide; a blue colour indicates the presence of iron. (d) Use the stain for the hsemin test.