The basis of radioimmunoassay.

London : University of London Audio-Visual Centre, 1972.

1 encoded moving image (38.01 min.) : sound, black and white.

00:38:01

University of London

Unrestricted

CC-BY-NC

Creative Commons Attribution-Non-Commercial 2.0 UK: England & Wales

In English

Presented by Professor John Landon, St Bartholomew's Hospital Medical College. Introduced by Dr Ian Gilliland. Produced by Peter Bowen. Directed by David Sharp. Made by University of London Audio-Visual Centre. Made for British Postgraduate Medical Federation.

This video is one of around 310 titles, originally broadcast on Channel 7 of the ILEA closed-circuit television network, given to Wellcome Trust from the University of London Audio-Visual Centre shortly after it closed in the late 1980s. Although some of these programmes might now seem rather out-dated, they probably represent the largest and most diversified body of medical video produced in any British university at this time, and give a comprehensive and fascinating view of the state of medical and surgical research and practice in the 1970s and 1980s, thus constituting a contemporary medical-historical archive of great interest. The lectures mostly take place in a small and intimate studio setting and are often face-to-face. The lecturers use a wide variety of resources to illustrate their points, including film clips, slides, graphs, animated diagrams, charts and tables as well as 3-dimensional models and display boards with movable pieces. Some of the lecturers are telegenic while some are clearly less comfortable about being recorded; all are experts in their field and show great enthusiasm to share both the latest research and the historical context of their specialist areas.

Segment 1 Gilliland introduces Landon. Landon introduces the subject of radioimmunoassay, describing what a powerful analytical tool it can be and giving some examples where it has been used successfully. Time start: 00:00:00:00 Time end: 00:04:57:00 Length: 00:04:57:00

Segment 2 Landon shows a chart which compares how many radioimmunoassay experiments occurred each year in 1968 compared with 1972. A brief film clip of the Technicon Autoanalyzer is shown as well as a photograph of a PDP-11 radioimmunoassay system. A further short film shows a protein-bound iodine machine at work in a laboratory and there is a film showing various mass spectrometers in a laboratory. Time start: 00:04:57:00 Time end: 00:10:33:00 Length: 00:05:36:00

Segment 3 Landon describes how bioassays in general are the best method for measuring hormones. He refers to several detailed charts which show how radioimmunoassays have been used effectively to measure various hormones. Time start: 00:10:33:00 Time end: 00:14:51:00 Length: 00:04:18:00

Segment 4 Landon describes the requirements for a radioimmunoassay and refers to a list of possible reagents. He then discusses the difficulties behind the radioimmunoassay technique. Time start: 00:14:51:00 Time end: 00:19:55:00 Length: 00:05:04:00

Segment 5 Landon lists the advantages of radioimmunoassay, referring to a slide containing this information. He shows the results of a radioimmunoassay tests for the hormones oxytocin and then deoxycorticosterone, progesterone and corticosterone. Time start: 00:19:55:00 Time end: 00:25:23:12 Length: 00:05:28:12

Segment 6 Landon describes the structural differences of various hormones, as detected by radioimmunoassay techniques. Time start: 00:25:23:12 Time end: 00:30:40:14 Length: 00:05:17:02

Segment 7 Landon summarises the lecture and discusses possible future developments for immunoassay techniques in general. Short films are shown of the chromato-electrofluoretic system and a laboratory filled with scientists at work on assays. Time start: 00:30:40:14 Time end: 00:38:01:24 Length: 00:07:21:10