Methods and formulae used in the preparation of animal and vegetable tissues for microscopical examination : including the staining of bacteria / by Peter Wyatt Squire.

Date:
1892
Catalogue details

Licence: Public Domain Mark

Credit: Methods and formulae used in the preparation of animal and vegetable tissues for microscopical examination : including the staining of bacteria / by Peter Wyatt Squire. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    STAINS. These may be divided into Nuclear, Plasmatic, or Specific, according to their jaower of staining the nuclei, the whole tissue, or certain elements of the tissue only. Nuclear Stains.—Hsematoxylin, Carmine and Cochineal, Methylene Blue, Methyl Green, Safranine, Vesuvine (Bis- marck Brown), Gentian Violet, Hoffmann's Blue (acidified), and Fuchsine. Plasmatic Stains.—Picric Acid, Benzopurpurine, Eosine, Erythrosine, Orange, and Acid Fuchsine. Specific Stains.—Osmic Acid, Chloride of Gold, Nitrate of Silver, Nigrosine, Acid Fuchsine, Congo Eed, Dahlia Violet, Methyl Violet, Iodine, Eose Bengale, Safranine, and Victoria Blue. Stains and methods for Micro-Organisms are treated separately. NUCLEAR STAINING. There is no colouring matter which is a nuclear stain only. All of them have more or less effect upon the ground sub- stance also. The value, therefore, of any dye as a nuclear stain depends upon the comparative affinity which it has for nuclei and ground substance respectively. Direct Nuclear Stains are those in which the nuclei are dyed so rapidly, compared with the rest of the tissue, that no particular treatment is necessary to differentiate them. Indirect Nuclear Stains are those in which the whole tissue is deeply stained, and the nuclei are differentiated by subsequent removal of the colour from the ground substance.