Standard methods of the Division of Laboratories and Research of the New York State Department of Health : General laboratory procedures and methods used ... / Augustus B. Wadsworth, director.

  • New York (State). Department of Health. Division of Laboratories and Research.
Date:
1927
Catalogue details

Licence: Public Domain Mark

Credit: Standard methods of the Division of Laboratories and Research of the New York State Department of Health : General laboratory procedures and methods used ... / Augustus B. Wadsworth, director. Source: Wellcome Collection.

    42/754 (page 16)
    determined also by means of the stage micrometer. With this type of instrument, accurate measurements can be made to a tenth of a micron. Warm Stage A warm stage, as the name indicates, is a device for holding material which is being examined with the microscope, at a temperature above that of the room. It is most commonly used in the examination of feces for protozoa and to study development of bacterial or other cells. The warm stage fits on the stage of a microscope leaving a hole in the center to admit the light. It is usually heated by means of electricity but warm water is used in some of the older types. Never, under any circumstances, attempt to repair a microscope. Report any injury or difficulty to the bacteriologist in charge of the group. PREPARATION OF STAINS AND REAGENTS GENERAL Acid Alcohol.— Hydrochloric acid): CuPis)u aj ispioc sisi eee abe aie aaa cman 3.00 cc. Alcohol, .95. per cent. ..323 4:64 ae ae alee ue ee 97.00 cc. Albert’s Solution No. 1.— | ohardin blues ty sissekes era bes Gh tales oie ca egal t e 0.15 gram Methyl greens: oc.) ais va tae ald a eee ee 0.20 gram Glacial acetic acid: 20). 206 ae eal 1.00 ce. Alcohol):.95 per cent s/s) 5077 ae a ee 2.00 cc. Distilledswatersvithes xc eiates el tle eer eameiaes 100.00 ce. Mix well, let stand 24 hours and filter. Albert’s Solution No. 2.— PONG foci Wehbe race Be eualiy band ie oN cee 2.00 grams Potassmmraodide tic; 5 .dve Acoskcs ap caelemialee aie ae 3.00 grams Distilled water. ki 4 nse he 300.00 cc. Grind the iodine crystals and the potassium iodide in a mortar until thoroughly mixed. Add the water slowly. Ammonium Ferric Sulfate Solution (Iron Alum).— Ammonium ferrigisulfate. 6 Oe dies eels ee 2.00 grams Distilled waters We idicde caved ccihvshe ale Mel hal ee meee: 98.00 ce. Anilin Gentian Violet.— Ariilin, obbc5 id cashes ahccre:, tka rad: tk 2 Se i re a Ce 1.00 ce. Dhatilled waiter. ade wavahttetiss hast sisces> ils SMA ca a prea 25.00 ce. Shake well in a bottle and filter through hard filter paper, moistened