Chemical examination of the barks of Brucea antidysenterica, Lam., and Brucea sumatrana, Roxb / by Arthur H. Salway and Walter Thomas.

  • Salway, Arthur H. (Arthur Henry)
Date:
[1907?]
Catalogue details

Licence: In copyright

Credit: Chemical examination of the barks of Brucea antidysenterica, Lam., and Brucea sumatrana, Roxb / by Arthur H. Salway and Walter Thomas. Source: Wellcome Collection.

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    could be isolated from it. The liquid gave with ferric chloride a green coloration, indicating the presence of tannin. Filtrate from Basic Lead Acetate Precipitate■,—This was treated with hydrogen sulphide for the removal of the lead, the mixture filtered, and the filtrate concentrated under diminished pressure. The light-brown syrupy liquid thus obtained still possessed a very bitter taste, and readily reduced Fchling’s solution on heating, but gave no precipitate with tannic acid, and only a faint brown coloration with ferric chloride. With phenylhydrazine acetate it yielded a crystalline osazone, which melted at 216°C., and therefore evidently contained a considerable amount of glucose. (B) The Resins. The resinous substances, which had been separated from the aqueous liquid, as above described, formed a brittle, black, amorphous solid. This was dissolved in a little alcohol, mixed with prepared sawdust, and the thoroughly dried mixture then extracted successively in a Soxhlet apparatus, with the following solvents :— Petroleum (b.p. 35-50°C.) . . extracted 9-90 Gm. 4-40 „ 3- 00 „ 0-45 ,, 4- 80 „ Total . . 22-55 Gm. The portion of resin extracted by the light petroleum was a soft, dark-coloured, wax-like mass. It was heated with an alco¬ holic solution of potassium hydroxide, the alcohol then removed, water added, and the mixture extracted with ether. The ethereal liquid yielded about 1 Gm. of a solid substance, which, after one crystallization from ethyl acetate containing a little dilute alcohol, separated in large flat plates, melting at 133°C. This substance, when dissolved in chloroform, gave on the addition of a little acetic anhydride and a drop of concentrated sulphuric acid the colour reaction characteristic of the phytosterols. It was analysed, with the following result:— 0 5161, when heated at 110 C., lost 0 0269 H2(). H20=5 2. 0 1503 of anhydrous substance gave 0 4555 C02 and 01614H20. C=82 7, H = 119 C2oH340:H„0 requires H20=5*8 per cent. C20H3lO requires c=82'8, H = 11 7 per cent. Chloroform Ethyl Acetate Alcohol .