Chemical examination of wheat germ / by Frederick B. Power and Arthur H. Salway.

  • Power, Frederick B. (Frederick Belding), 1853-1927
Date:
[1913?]
Catalogue details

Licence: Public Domain Mark

Credit: Chemical examination of wheat germ / by Frederick B. Power and Arthur H. Salway. Source: Wellcome Collection.

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    Examination of the Aqueous Liquid. The aqueous liquid from which the fatty oil and resin had been separated, as above described, was extracted many times with ether. This solvent removed about 3 grammes of viscid, fatty material, which was evidently similar in character to the main portion of fatty oil. The aqueous liquid was next shaken repeatedly with hot amyl alcohol. The amyl alcohol liquids were then united, washed with a little water, and the solvent removed by dis¬ tillation under diminished pressure, when a quantity (10 grammes) of a dark brown syrup was obtained. This was dis¬ solved in alcohol and the solution kept for some time, when a small quantity of a crystalline solid separated. The latter was collected and dissolved in hot dilute alcohol, from which it was deposited in slender, colourless needles, melting and decomposing at about 240°. The same compound was subse¬ quently isolated in larger amount from the aqueous liquid, as described below, and was then completely identified as allantoin. The alcoholic liquid from which the small amount of allan¬ toin had first separated, as above noted, was evaporated, and the residue divided into two portions. One portion was heated for some time with dilute sulphuric acid, when a small amount of a sugar was formed, which yielded an osazone melting at 210°, but no other definite hydrolytic pro¬ duct was obtained. It was thus evident, however, that the material was glucosidic'in character. Isolation of Sinapic Acid, (4-Hydroxy-3: 5-dimethoxycinnamic Acid) C6H2OH-(OCH3)2. CH : CH C02H. The remaining portion of the residue from the above- mentioned alcoholic liquid was heated for a few minutes with an excess of aqueous potassium hydroxide, the mixture then acidified with dilute sulphuric acid, and subsequently extracted with ether. After washing and drying the ethereal liquid, the solvent was removed, when about 0'5 gramme of a brown, crystalline solid remained. This was re- dissolved in ether, the solution shaken with aqueous ammo¬ nium carbonate, and the alkaline liquid acidified and ex¬ tracted with ether, when a crystalline substance was ob¬ tained, which melted and decomposed at about 186°. After recrystallisation from dilute alcohol, it separated in nearly