Chemical examination of the roots of phaseolus multiflorus / by Frederick B. Power and Arthur H. Salway.

  • Power, Frederick B. (Frederick Belding), 1853-1927.
Date:
[1913?]
Catalogue details

Licence: Public Domain Mark

Credit: Chemical examination of the roots of phaseolus multiflorus / by Frederick B. Power and Arthur H. Salway. Source: Wellcome Collection.

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    mixture of ethyl acetate and alcohol, when it separated in colourless plates, and was analysed. 0 0648 gave 0 1992 C02 and 0 0712 HJJ. C = 838; H = 122. C27H160 requires C = 83'9 ; H = 11'9 per cent. The substance is thus seen to agree in composition with a phytosterol, and it gave the colour reaction of that class of compounds. The aqueous alkaline solution of potassium salts resulting from the hydrolysis of the petroleum extract of the resin, after having been shaken with ether to remove the unsaponi liable material, as above described, was acidified with dilute sul¬ phuric acid, when a precipitate of fatty acids was produced. On extracting the mixture with ether it was observed that a small portion of the precipitate was very sparingly soluble in that liquid. This material was, therefore, collected and crystallised from dilute pyridine, when it separated in felted masses of colourless plates, melting at 275°. When dissolved in acetic anhydride and chloroform, and a few drops of con¬ centrated sulphuric acid added, a transient pink colouration, rapidly changing to blue, and then to green, was produced. I1 he substance thus possessed the characters of a phyto- sterolirC (phytosterol glucoside), but the amount available was not sufficient for analysis or for ascertaining its homogeneity. It yielded an acetyl derivative melting at 162°. Examination of the Fatty Acids. The ethereal solution of fatty acids, obtained as described above, yielded 5 grammes of a semi-solid product, which was distilled under diminished pressure, when 4 grammes passed over at 250-265°/60 Mm., leaving a non-volatile, dark-coloured residue. The distilled acids were then separated into solid and liquid portions 'by the usual method of conversion into their lead salts, and treatment of the latter with ether. The Solid Acids.—'These amounted to about 1 gramme. After one crystallisation from alcohol they melted at 55°, and gave the following result on analysis :— 0T167 gave 0-3274 C02 and 0T312 H,0. C = 76 5 ; II = 12 5. C18H;1602 requires C = 761 ; H = 12 7 per cent. C20H40O2 requires C = 76 9 ; H = 12 8 per cent. The melting-point and analysis of the above product clearly indicated it to be a mixture of fatty acids, but their separa¬ tion could not be effected with the small amount of material available. The Liquid Acids.—These acids amounted to about 2 grammes. When distilled under diminished pressure they passed over at 220-240°/18 Mm. as a yellow oil. An analysis and a determination of the iodine value gave the following results :— 0T099 gave 0 3070 CO, and 0T120 11,0. C - 76 2 ; II - 113. 0T734 absorbed 6-2583 Iodine. Iodine value — 149. Cmll^O, requires C = 76 6; II = 121 per cent. I.V. — 90T. ClhH;(iO, requires C = 771; H = 11-4 per cent, I.V. = 181-4.