Non-HLA antigens in health, aging, and malignancy : proceedings of a conference held at Roswell Park Memorial Institute, Buffalo, New York, March 28 and 29, 1983 / editors, Elias Cohen, Dharam P. Singal.

Date:
[1983], ©1983
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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Non-HLA antigens in health, aging, and malignancy : proceedings of a conference held at Roswell Park Memorial Institute, Buffalo, New York, March 28 and 29, 1983 / editors, Elias Cohen, Dharam P. Singal. Source: Wellcome Collection.

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    Immunodiagnosis of Pancreatic Cancer / 177 than those obtained with crude extracts (range -0.20 to -0.30). More recently we have attempted to increase the yield of the acidic protein by substituting ion exchange chromatography (CM Sephadex C-25) for the Sepharose 6B step. This has resulted in an antigen that is non- specifically reactive in the microplate LAI assay (unpublished observations). Analysis of this material by Polyacrylamide gel electrophoresis has revealed the presence of several large molecular weight components. The additional components may account for the nonspecific reactions. Thus we are returning to gel filtration chromatography as our final step. A xenogeneic antiserum to the middle fraction was generated in a goat by established procedures. After absorption, this antiserum was able to detect the antigen in 8 of 11 effusion fluids from patients with pancreatic cancer and 1 of 6 control effusion fluids by immunodiffusion (Campagnari et al). Preliminary studies of the localization of xenogeneic antiserum on deparaffinized tissue sections of pancreas carcinoma and adjacent normal pancreas have been performed by an indirect immunoperoxidase procedure (DeLellis et al 1979). The xenogeneic antiserum selectively localized to the malignant ductal epithelial cells of the pancreas and small intestine but not to the normal ductal epithelial cells. The patterns of staining observed with malignant and normal pancreatic ductal epithelial cells is depicted below. Figure 2A