Report of experiments now being pursued in the Bacteriological Laboratory of the Academy / by Samuel G. Dixon.

  • Dixon, Samuel Gibson, 1851-1918.
Date:
1891
Catalogue details

Licence: Public Domain Mark

Credit: Report of experiments now being pursued in the Bacteriological Laboratory of the Academy / by Samuel G. Dixon. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    PKo<'KKI)[\(;s ok THi: ATADDMV (,|- | | K<) | The tuberculous mass is first placed in n mortar and then fi„ely chopped up with a pair of shears. Then it is thoroughly mashed with a pestle, and treated with water, or better still with water and glycerine, for twelve hours at a low temperature. It has been my habit, however, to expose it to a temperature of 40° C. for twelve hours longer; after which the substance is poured into a Chamber- lain-Pasteur filtering tube, which it is permitted to slowly pass through without pressure. Its effect on tuberculous animals would seem to be about as repre- sented by the following reaction :— Tuberculous Cow. Before inoculation temperature 101° F. 3 cc. of filtrate was subcutaneously injected at 10 a. m. At 5 p. m. temperature was 102°; at 10 p. m. 103°; at 12 p. m. 103!°; at 10 a. in. 10U°. Guinea Pigs.1 No. 3-A. At 11 a. m. the temperature was 101!°; at 1 p. m. 101° ; at 3 p. m. 101°. At 2.20 p. m. ]-20 cc. of tubercu- lous agent from lung was subcutaneously injected. At 5.45 the temperature was 10H°; at 7 p.m. 102°; at 11 p.m. 101t° ; at 1.30 a. m. 10H°; at 8 a. m. 101 f ° ; at 12.30 p. m. 100P ; at 3 p. m. 1011°. No. 4-A. At 11 a. in. 10U°; at 1 p. m. 1013° ; at 3 p. m. 1011° : At this time 1-10 cc. toxic agent from lung of a cow subcutane- ously injected. At 5.45 p. m. temperature 102£° ; at 7 p. m. 103i°; at 11 p. m. 102f° ; at 1.30 p. m. 10U° ; at 8 a. m. 101 *° ; at 12.30 p. m. 102A° ; at 3 p. m. 1011°. In addition to my last report of investigations I have to refer to the treatment of tuberculosis by subcutaneous injections of dilute sulphuric acid and formic acid. Under each of these methods the animals would appear to be doing better than the check guinea pigs not so treated, yet sufficient time has not elapsed for me to make any post-mortems to determine the processes going on in the vis- cera. The points of inoculation, however, have healed beautifully and the animals would appear to be doing well. The experiments towards the securing of immunity are still being conducted and the results certainly show that the animal organism can lie cultivated to resist inoculations of the tubercle bacillus to a greater or less 1 Temperature in the Guinea pig is not constant; and therefore not as reliable for experimentation as either the cow or dog.